!IV.5. Dimethylphosphate-Sodium complex with freely moving sodium !This input show how to allow tranlational/rotational degrees of freedom !for molecules considered 'solute' by the program. FILE dmpna TITL Na+DMP-: Kollman-Straatsma(Na+) TITL SPC water. H-R PBC HRDW VC32 ! 32-bit vector algorithm SVVC SPCC 7.75 ! Solvent spherical cutoff SUVC MIGC 0.0 ! MI on the solute based on group center distances SUUC MIMC ! Molec-based MI for intrasolute PBCN RECT 32.031 21.354 21.354 ! Rectangular PBC, long box TEMP 298 | NSLV 485 !Simulation at 298 Kelvin with 485 solvent molecules STEP 0.1 0.0 0.55 40.0 10 ! 0 slt stepsize, 0.55 Å, 40 deg solvent steps SVPT TIP3 TIP3 ! Solvent-solvent pot: TIP3P SUPT AM94 ! Solute-solvent pot: AMBER SAMP FBSC 0.5 ! Scaled force-biased sampling 2 4.00 0.2 7.0 1.0 MOVE PRSP NRST ! Preferential sampling based on nearest slt atom dist 3 3.00 3.5 4.0 03.0 7.0 1.0 PMOD AM94 1 ! Potential library modification 49 46 11 1.59955E+00 0.478833E-01 ! Solute's "own" solvent will be used for slt-slv SLTA SMPL MMC READ 8 ! 8 solute atom - regular solute, read from input stream 38 0.0 0.0 0.0 0.91200 1 35 0.74000 0.0 1.28171 -0.65500 1 35 0.74000 0.0 -1.28171 -0.65500 1 37 -0.99164 -1.25560 0.00000 -0.41000 1 37 -0.99164 1.25560 0.00000 -0.41000 1 4 -1.92075 1.43300 1.07251 0.10900 1 4 -1.92075 -1.43300 -1.07251 0.10900 1 49 2.70000 0.0 -0.00000 1.00000 2 PARD UNIF SHCY 1.0 0.3 0.0 1.0 0.3 0.0 ~ 1.0 0.3 0.0 1.0 -1 ! Last slt group is independently moved DSTC NONE ! No distribution function calc SLFT BASC ! Basic self tests performed CNFG READ ASCI ! Input from dmpna.crd in ASCII RUNS 2000000 50000 1000000 0100000 100000 ! Run 2M steps STOP !Files needed for run: !dmpna.crd: initial configuration. !Files created by the run: ! * dmpna.ckp: checkpoint file after the first run; ! * dmpna.2.crd: final configuration after the run.