!IV.10. Widom insertion method !This input shows how to run a Widom insertion calculation based on a !pure water trajectory run earlier by CHARMM. FILE methane PRNT ECHO TITL Calculate the chemical potential of Methane in Water TITL using Widom cavity insertion method HRDW VC32 ! 32-bit vector SVVC MINI 10.0 SUVC MIGC 10.0 ! MI on the solute PBCN RECT 15.5 15.5 15.5 !Rectangular PBC TEMP 300 NSLV 124 !no of solvent molecs in the CHARMM run SVPT TIP4 TIP4 ! Solvent-solvent potential is TIP4P SUPT CHRM !Solute-solvent potential is CHARMM FREE WIDO 2.6 64 64 64 2 5 SLTA SMPL MMC READ 5 5 CT3 -0.13265 0.14073 0.03004 -0.36000 1 CSP3C1 HA 0.84914 0.15299 0.45527 0.09000 1 CSP3H11 HA -0.35123 -0.84076 -0.33574 0.09000 1 CSP3H12 HA -0.84914 0.40992 0.77775 0.09000 1 CSP3H13 HA -0.18078 0.84077 -0.77776 0.09000 1 CSP3H14 TRAJ CHRM RGFX !Open trajectory in CHARMM format SCAN TRAJ 1000 5 00 0 500 2 1 2 3 1 STOP !File needed for run: ! * methane.hst: the CHARMM trajectory (DCD) file !Files created by the run: ! * methane.ckp: checkpoint files ! * methane.pxc: analysis checkpoint file